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Rabbit Anti-Big endothelin-1  antibody (bs-0202R)  
~~~促銷,代碼KT202502A~~~
~~~促銷,代碼KT202502B~~~
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產(chǎn)品編號 bs-0202R
英文名稱 Rabbit Anti-Big endothelin-1  antibody
中文名稱 內(nèi)皮素1抗體
別    名 EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1.  
Specific References  (1)     |     bs-0202R has been referenced in 1 publications.
[IF=3.647] Juan Wang. et al. NR4A3 induces endothelial dysfunction through up-regulation of endothelial 1 expression in adipose tissue-derived stromal cells. Life Sci. 2021 Jan;264:118727  IF ;  Human.  
研究領(lǐng)域 心血管  免疫學(xué)  神經(jīng)生物學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22kDa
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Big endothelin-1: 81-212/212 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.

Function:
Endothelins are endothelium-derived vasoconstrictor peptides.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.

Similarity:
Belongs to the endothelin/sarafotoxin family.

SWISS:
P05305

Gene ID:
1906

Database links:

Entrez Gene: 1906?Human

Entrez Gene: 13614?Mouse

Entrez Gene: 24323?Rat

Omim: 131240?Human

SwissProt: P05305?Human

SwissProt: P22387?Mouse

SwissProt: P22388?Rat

Unigene: 511899?Human

Unigene: 713645?Human

Unigene: 10918?Rat



內(nèi)皮素(Endothelin-1,ET)是一種強的血管收縮劑,分為ET-1,ET-2,ET-3 三種,ET-1主要在內(nèi)皮細胞中表達,而ET-2和ET-3則主要在神經(jīng)系統(tǒng)中表達。
ET具有廣泛的生物學(xué)作用.
產(chǎn)品圖片
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Testis (Rat) Lysate at 40 ug Primary: Anti-Big endothelin-1 (bs-0202R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD
Tissue/cell: human gastric mucosa; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ET-1 Polyclonal Antibody, Unconjugated(bs-0202R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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