| 產(chǎn)品編號 | bs-2082R |
| 英文名稱 | Rabbit Anti-phospho-Caldesmon (Ser789) antibody |
| 中文名稱 | 磷酸化鈣介質(zhì)素抗體 |
| 別 名 | Caldesmon (phospho-S789); Caldesmon (phospho-Ser789); p-Caldesmon (S789); p-Caldesmon (Ser789); p-Caldesmon (phospho-Ser789); CAD; CALD 1; CALD1; CALD1_HUMAN; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22. |
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Specific References (1) | bs-2082R has been referenced in 1 publications.
[IF=0.939] Prayitnaningsih et al. Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. (2016) Int.J.Ophthalmol. 9:1568-1577 IF(IHC-P) ; Rat.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 心血管 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 93kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Caldesmon around the phosphorylation site of Ser789: VT(p-S)PT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jul 2008]. Function: Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, myofibril. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). Tissue Specificity: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. Post-translational modifications: In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity). Similarity: Belongs to the caldesmon family. SWISS: Q05682 Gene ID: 800 Database links: Entrez Gene: 800 Human Entrez Gene: 109624 Mouse Omim: 114213 Human SwissProt: Q05682 Human SwissProt: Q8VCQ8 Mouse Unigene: 490203 Human |
| 產(chǎn)品圖片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti- phospho-Caldesmon (Ser789) (bs-2082R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 93 kD
Observed band size: 86 kD
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