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Rabbit Anti-PNMT  antibody (bs-3912R)  
~~~促銷,代碼KT202502A~~~
~~~促銷,代碼KT202502B~~~
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說明書: 50ul  100ul  200ul
50ul/1180.00元
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200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-3912R
英文名稱 Rabbit Anti-PNMT  antibody
中文名稱 苯乙醇胺甲基轉(zhuǎn)移酶抗體
別    名 Noradrenaline N-methyltransferase; PENT; Phenylethanolamine N-methyltransferase; PNMTase; PNMT_HUMAN.   
Specific References  (3)     |     bs-3912R has been referenced in 3 publications.
[IF=14.612] Na Zhou. et al. Specific Fluorescent Probe Based on “Protect–Deprotect” To Visualize the Norepinephrine Signaling Pathway and Drug Intervention Tracers. J Am Chem Soc. 2020;142(41):17751–17755  IF ;  Human.  
[IF=6.35] Yue Y et al. Noradrenaline-Specific,?Efficient?Visualization?in?Brain?Tissue?Triggered?by?Unique?Cascade Nucleophilic?Substitution. Anal Chem.?2019 Feb 5;91(3):2255-2259.  IHF ;  Mouse.  
[IF=3.58] Kawahata, Ichiro, and Tohru Yamakuni. "Imidacloprid, a neonicotinoid insecticide, facilitates tyrosine hydroxylase transcription and phenylethanolamine N-methyltransferase mRNA expression to enhance catecholamine synthesis and its nicotine-evoked elevation in PC12D cells." Toxicology (2017).  Rat.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Rabbit,Pig,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31kDa
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PNMT: 101-200/282 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Phenylethanolamine N-methyltransferase, PNMT, catalyzes the synthesis of epinephrine from norepinephrine, (adrenalin from noradrenalin), in catecholamine biosynthesis.

Function:
Converts noradrenaline to adrenaline.

Similarity:
Belongs to the NNMT/PNMT/TEMT family.

SWISS:
P11086

Gene ID:
5409

Database links:
UniProtKB/Swiss-Prot: P11086.1

PNMT又稱去甲腎上腺素N-甲基轉(zhuǎn)移酶,其作用;催化將甲基從S-腺苷酰甲硫氨酸轉(zhuǎn)移到去甲腎上腺素的氨基端生成腎上腺素反應(yīng)的酶。一般認(rèn)為:嗜鉻細(xì)胞胞漿中存在大量的PNMT,可使去甲腎上腺素甲基化而成腎上腺素(另一個生成腎上腺素的方式)。
產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PNMT) Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PNMT) Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PNMT) polyclonal Antibody, Unconjugated (bs-3912R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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