| 產(chǎn)品編號 | bs-3912R |
| 英文名稱 | Rabbit Anti-PNMT antibody |
| 中文名稱 | 苯乙醇胺甲基轉(zhuǎn)移酶抗體 |
| 別 名 | Noradrenaline N-methyltransferase; PENT; Phenylethanolamine N-methyltransferase; PNMTase; PNMT_HUMAN. |
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Specific References (3) | bs-3912R has been referenced in 3 publications.
[IF=14.612] Na Zhou. et al. Specific Fluorescent Probe Based on “Protect–Deprotect” To Visualize the Norepinephrine Signaling Pathway and Drug Intervention Tracers. J Am Chem Soc. 2020;142(41):17751–17755 IF ; Human.
[IF=6.35] Yue Y et al. Noradrenaline-Specific,?Efficient?Visualization?in?Brain?Tissue?Triggered?by?Unique?Cascade Nucleophilic?Substitution. Anal Chem.?2019 Feb 5;91(3):2255-2259. IHF ; Mouse.
[IF=3.58] Kawahata, Ichiro, and Tohru Yamakuni. "Imidacloprid, a neonicotinoid insecticide, facilitates tyrosine hydroxylase transcription and phenylethanolamine N-methyltransferase mRNA expression to enhance catecholamine synthesis and its nicotine-evoked elevation in PC12D cells." Toxicology (2017). Rat.
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| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PNMT: 101-200/282 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Phenylethanolamine N-methyltransferase, PNMT, catalyzes the synthesis of epinephrine from norepinephrine, (adrenalin from noradrenalin), in catecholamine biosynthesis. Function: Converts noradrenaline to adrenaline. Similarity: Belongs to the NNMT/PNMT/TEMT family. SWISS: P11086 Gene ID: 5409 Database links: UniProtKB/Swiss-Prot: P11086.1 PNMT又稱去甲腎上腺素N-甲基轉(zhuǎn)移酶,其作用；催化將甲基從S-腺苷酰甲硫氨酸轉(zhuǎn)移到去甲腎上腺素的氨基端生成腎上腺素反應(yīng)的酶。一般認(rèn)為：嗜鉻細(xì)胞胞漿中存在大量的PNMT，可使去甲腎上腺素甲基化而成腎上腺素（另一個生成腎上腺素的方式）。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PNMT) Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PNMT) Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PNMT) polyclonal Antibody, Unconjugated (bs-3912R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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