| 產(chǎn)品編號(hào) | bs-2373R |
| 英文名稱(chēng) | Rabbit Anti-Ceruloplasmin antibody |
| 中文名稱(chēng) | 銅藍(lán)蛋白抗體 |
| 別 名 | CER; caeruloplasmin; Ceruloplasmin precursor; CP 2; CP; CP2; CP-2; EC 1.16.3.1; Ferroxidase; CERU_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 120kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ceruloplasmin: 551-650/1065 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Caeruloplasmin is a copper binding plasma glycoprotein consisting of a single polypeptide chain. It has a molecular weight of 132kDa and occurs in plasma at a concentration of 150-500 mg/L. Although the physiological functions of Caeruloplasmin are not fully understood it is implicated in a number of possible functions. 95% of plasma copper is bound to Caeruloplasmin, implying some involvement in copper transport. Caeruloplasmin has been described as both an oxidant and an antioxidant, its exact role or roles in this respect are not clear. Plasma levels of Caeruloplasmin are elevated after inflammation and trauma and it is therefore classified as an acute phase protein. The measurement of Caeruloplasmin in plasma is useful in the diagnosis of Wilson’s disease, where levels are greatly reduced. Caeruloplasmin is extremely labile in plasma and rapidly degrades to 115kDa and 19kDa fragments. The age of plasma samples should therefore be taken into account when interpreting immunological analysis of caeruloplasmin. Function: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Subcellular Location: Secreted. Note=Colocalizes with GCP1 in secretory intracellular compartments. Tissue Specificity: Expressed by the liver and secreted in plasma. DISEASE: Aceruloplasminemia (ACERULOP) [MIM:604290]: An autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Note=The disease is caused by mutations affecting the gene represented in this entry. Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Similarity: Belongs to the multicopper oxidase family. Contains 3 F5/8 type A domains. Contains 6 plastocyanin-like domains. SWISS: P00450 Gene ID: 1356 Database links: Entrez Gene: 1356 Human Entrez Gene: 12870 Mouse Omim: 117700 Human SwissProt: P00450 Human SwissProt: Q61147 Mouse Unigene: 558314 Human Unigene: 13787 Mouse ????銅藍(lán)蛋白（ceruloplasmin,CER）又稱(chēng)銅氧化酶，是一種含銅的α2糖蛋白，CER具有氧化酶的活性，起著抗氧化劑的作用。在血循環(huán)中CER的抗氧化活力可以防止組織中脂質(zhì)過(guò)氧化物和自由基的生成，特別在炎癥時(shí)具有重要意義,也屬于一種急性時(shí)相反應(yīng)蛋白。 ????銅藍(lán)蛋白對(duì)多酚及多胺類(lèi)底物有催化其氧化之能力銅藍(lán)蛋白具有遺傳上的基因多形性。其作用為調(diào)節(jié)銅在機(jī)體各個(gè)部位的分布、合成含銅的酶蛋白.一般認(rèn)為銅藍(lán)蛋白由肝臟合成，一部分由膽道排泄，尿中含量甚微。銅藍(lán)蛋白測(cè)定對(duì)某些肝、膽、腎等疾病的診斷有一定意義。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Serum tissue lysates
Lane 2: Rat Serum tissue lysates
Primary: Anti-Ceruloplasmin (bs-2373R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kDa
Observed band size: 140 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceruloplasmin) Polyclonal Antibody, Unconjugated (bs-2373R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
