| 產(chǎn)品編號 | bs-4248R |
| 英文名稱 | Rabbit Anti-ORP150 antibody |
| 中文名稱 | 氧氣調節(jié)蛋白150抗體 |
| 別 名 | 150 kDa oxygen regulated protein; 150 kDa oxygen-regulated protein; 170 kDa glucose regulated protein; 170 kDa glucose-regulated protein; DKFZp686N08236; FLJ94899; FLJ97572; glucose regulated protein 170; Grp 170; GRP-170; Grp170; HSP 12A; HSP12A; Hyou1; HYOU1_HUMAN; Hypoxia up regulated 1; hypoxia up-regulated 1 precursor; Hypoxia up-regulated protein 1; Hypoxia upregulated 1; Orp 150; ORP-150; Orp150; oxygen regulated protein (150kD). |
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Specific References (1) | bs-4248R has been referenced in 1 publications.
[IF=2.12] Ruan Lin, et al. "Tetrandrine Attenuated Cerebral Ischemia/Reperfusion Injury and Induced Differential Proteomic Changes in a MCAO Mice Model Using 2-D DIGE." Neurochemical Research (2013). 1-9. WB ; Rat.
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| 研究領域 | 腫瘤 心血管 免疫學 染色質和核信號 神經(jīng)生物學 信號轉導 新陳代謝 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 108kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ORP150: 401-500/999 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ORP150 (150KDa Oxygen-regulated Protein) is a member of the heat shock protein/stress protein family and has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. This protein has also been described as a molecular chaperone, induced by various environmental and physiological stimuli, and participates in protein folding in the endoplasmic reticulum. Furthermore, ORP150 is up-regulated in tumors and, in breast tumors, may be associated with tumor invasiveness. Function: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Subunit: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Subcellular Location: Endoplasmic reticulum lumen. Tissue Specificity: Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. Similarity: Belongs to the heat shock protein 70 family. SWISS: Q9Y4L1 Gene ID: 10525 Database links: Entrez Gene: 10525 Human Entrez Gene: 12282 Mouse Omim: 601746 Human SwissProt: Q9Y4L1 Human SwissProt: Q9JKR6 Mouse Unigene: 277704 Human |
| 產(chǎn)品圖片 |
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- ORP150 (bs-4248R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kD
Observed band size: 108 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
