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Rabbit Anti-KLST/Kallistatin  antibody (bs-5786R)  
~~~促銷,代碼KT202502A~~~
~~~促銷,代碼KT202502B~~~
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產(chǎn)品編號(hào) bs-5786R
英文名稱 Rabbit Anti-KLST/Kallistatin  antibody
中文名稱 激肽釋放酶抑制劑抗體
別    名 Serpin peptidase inhibitor clade A member 4; Kallikrein binding protein; Kallikrein inhibitor; Protease inhibitor 4; Serpin A4; KAL; Kallikrein binding protein; Kallikrein inhibitor; KBP; KLST; KST; MGC108582; PI4; Protease inhibitor 4; Serpin A4; Serpin peptidase inhibitor clade A member 4; SERPINA4; KAIN_HUMAN.  
Specific References  (1)     |     bs-5786R has been referenced in 1 publications.
[IF=2.2] Biswas et al. Polymorphisms at the F12 and KLKB1 loci have significant trait association with activation of the renin-angiotensin system. (2016) BMC.Med.Genet. 17:21  IF ;  Mouse.  
研究領(lǐng)域 心血管  細(xì)胞生物  免疫學(xué)  生長(zhǎng)因子和激素  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse (predicted: Human,Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 49kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KLST/Kallistatin: 151-250/427 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Kallistatin is a serine proteinase inhibitor which binds to tissue kallikrein and inhibits its amidolytic and kininogenase activity. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.

Function:
Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Subunit:
Monomer and some homodimers.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed by the liver and secreted in plasma.

Post-translational modifications:
The N-terminus is blocked.

Similarity:
Belongs to the serpin family.

SWISS:
P29622

Gene ID:
5267

Database links:

Entrez Gene: 5267 Human

Omim: 147935 Human

SwissProt: P29622 Human

Unigene: 719893 Human



產(chǎn)品圖片
Sample: Lane 14: Pancreas (Mouse) Lysate at 40 ug Lane 14: Kidney (Mouse) Lysate at 40 ug Lane 14: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-KLST/Kallistatin (bs-5786R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-60 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLST) Polyclonal Antibody, Unconjugated (bs-5786R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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