| 產(chǎn)品編號 | bs-7556R |
| 英文名稱 | Rabbit Anti-ALOX5AP antibody |
| 中文名稱 | 5脂氧合酶激活蛋白抗體 |
| 別 名 | 5-lipoxygenase activating protein; Arachidonate 5 lipoxygenase activating protein; 5-lipoxygenase activating protein; AL5AP_HUMAN; ALOX 5AP; ALOX5 AP; Arachidonate 5-lipoxygenase-activating protein; Five lipoxygenase activating protein; FLAP; MK 886 binding protein; MK-886-binding protein. |
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Specific References (2) | bs-7556R has been referenced in 2 publications.
[IF=8.78] Elias, Ivet, et al. "Alox5ap overexpression in adipose tissue leads to LXA4 production and protection against diet-induced obesity and insulin resistance." Diabetes 65.8 (2016): 2139-2150. WB ; Mouse.
[IF=7.27] Elias et al. ALOX5AP Overexpression in Adipose Tissue Leads to LXA4 Production and Protection Against Diet-Induced Obesity and Insulin Resistance. (2016) Diabetes. 65:2139-50 WB ; Mouse.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALOX5AP: 65-161/161 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Involvement in disease: Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) ; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Function: Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Subunit: Homotrimer. Interacts with LTC4S and ALOX5. Subcellular Location: Nucleus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. DISEASE: Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Similarity: Belongs to the MAPEG family. SWISS: P20292 Gene ID: 241 Database links: Entrez Gene: 241 Human Omim: 603700 Human SwissProt: Q2PG08 Cynomolgus Monkey SwissProt: P30353 Horse SwissProt: P20292 Human SwissProt: P30354 Macaque Monkey SwissProt: P30357 Rabbit Unigene: 507658 Human |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLAP Polyclonal Antibody, Unconjugated(bs-7556R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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