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Rabbit Anti-phospho-TIRAP (Tyr86)  antibody (bs-7562R)  
~~~促銷,代碼KT202502A~~~
~~~促銷,代碼KT202502B~~~
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產(chǎn)品編號 bs-7562R
英文名稱 Rabbit Anti-phospho-TIRAP (Tyr86)  antibody
中文名稱 磷酸化白細胞介素1受體銜接蛋白抗體
別    名 TIRAP (phospho Y86); Adapter protein wyatt; MAL antibody MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt.  
Specific References  (1)     |     bs-7562R has been referenced in 1 publications.
[IF=4.52] Rolf, Nina, et al. "Heterodimer‐specific TLR2 stimulation results in divergent functional outcomes in B‐cell precursor acute lymphoblastic leukemia."European journal of immunology (2015).  Human.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 心血管  細胞生物  免疫學  信號轉(zhuǎn)導  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Pig,Cow,Dog,GuineaPig,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 24kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human TIRAP around the acetylation site of Tyr86: KD(p-Y)DV 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production.

Function:
Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.

Subunit:
Homodimer. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to BMX, PKR and TBK1. Does not interact with IRAK1, nor TLR9.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.

Post-translational modifications:
Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK.
Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation.

Similarity:
Contains 1 TIR domain.

SWISS:
P58753

Gene ID:
114609

Database links:

Entrez Gene: 114609 Human

Entrez Gene: 117149 Mouse

Entrez Gene: 680127 Rat

GenBank: AF378129 Human

GenBank: AF378130 Human

Omim: 606252 Human

SwissProt: P58753 Human

SwissProt: Q99JY1 Mouse

Unigene: 537126 Human

Unigene: 23987 Mouse



產(chǎn)品圖片
Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HUVEC cell lysates Lane 3: Human U251 cell lysates Lane 4: Human SH-SY5Y cell lysates Lane 5: Human K562 cell lysates Primary: Anti-phospho-TIRAP (Tyr86) (bs-7562R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 35 kDa
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-TIRAP (Tyr86) ) Polyclonal Antibody, Unconjugated (bs-7562R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-TIRAP (Tyr86) ) Polyclonal Antibody, Unconjugated (bs-7562R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-TIRAP (Tyr86) ) Polyclonal Antibody, Unconjugated (bs-7562R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:U937. Primary Antibody (green line): Rabbit Anti-phospho-TIRAP(Tyr86) antibody (bs-7562R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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