產(chǎn)品編號 | bs-6850R |
英文名稱 | Rabbit Anti-TPST1 antibody |
中文名稱 | 酪蛋白硫酸轉(zhuǎn)移酶1抗體 |
別 名 | Protein tyrosine sulfotransferase 1; Protein-tyrosine sulfotransferase 1; TPST-1; TPST1_HUMAN; Tyrosylprotein sulfotransferase 1. |
研究領(lǐng)域 | 心血管 細(xì)胞生物 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 41kDa |
細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TPST1: 165-260/370 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. Function: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Similarity: Belongs to the protein sulfotransferase family. Gene ID: 8460 |
產(chǎn)品圖片 |
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-TPST1 (bs-6850R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 51 kD
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TPST1 Polyclonal Antibody, Unconjugated(bs-6850R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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