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Rabbit Anti-Galectin 12  antibody (bs-8413R)  
~~~促銷,代碼KT202502A~~~
~~~促銷,代碼KT202502B~~~
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產(chǎn)品編號(hào) bs-8413R
英文名稱 Rabbit Anti-Galectin 12  antibody
中文名稱 半乳糖凝集素12抗體
別    名 Galectin related inhibitor of proliferation; galectin-12; GRIP1; Lectin galactoside binding soluble 12 (galectin 12); LEG12_HUMAN.  
研究領(lǐng)域 心血管  細(xì)胞生物  免疫學(xué)  糖尿病  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=1ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 38kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Galectin 12/LGALS12: 101-200/336 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Galectin-12, also designated galectin-related inhibitor of proliferation 1 in mouse, is a 314 amino acid protein encoded by the human gene LGALS12. Galectin-12 is a member of the galectin family consisting of b-galactoside-binding proteins with conserved carbohydrate recognition domains. Galectin-12 binds lactose and may participate in the apoptosis of adipocytes. This protein is preferentially expressed in peripheral blood leukocytes and adipocytes. Galectin-12 is induced by cell cycle block at the G1 phase and causes G1 arrest when overexpressed. The galectin-12 gene is expressed in mouse preadipo-cytes and is upregulated when preadipocytes undergo cell cycle arrest, concomitant with acquisition of the competence to undergo differentiation in response to adipogenic hormone stimulation. Galectin-12 is an adipocyte-expressed protein which is downregulated by various insulin resistance-inducing hormones. As a result, galectin-12 may play a role in the pathogenesis of insulin resistance.

Function:
Binds lactose. May participate in the apoptosis of adipocytes.

Subcellular Location:
Nucleus.

Tissue Specificity:
Not widely expressed. Predominantly expressed in adipose tissue.

Similarity:
Contains 2 galectin domains.

SWISS:
Q96DT0

Gene ID:
85329

Database links:

Entrez Gene: 85329 Human

Omim: 606096 Human

SwissProt: Q96DT0 Human

Unigene: 502774 Human



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 12) Polyclonal Antibody, Unconjugated (bs-8413R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Galectin 12 Antibody(bs-8413R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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