| 產(chǎn)品編號 | bsm-52772R |
| 英文名稱 | Rabbit Anti-GPX1 antibody |
| 中文名稱 | 谷胱甘肽過氧化酶1重組兔單抗 |
| 別 名 | Glutathione peroxidase 1; Cellular glutathione peroxidase; GPx 1; GPx-1; GPX1; GPX1_HUMAN; GSHPx-1; GSHPX1; MGC14399; MGC88245. |
| 研究領(lǐng)域 | 腫瘤 心血管 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 8C4 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Glutathione Peroxidase 1 : 1-100/203 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene product belongs to the family of glutathione peroxidase, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq]. Subunit: Homotetramer. Interacts with MIEN1 (By similarity). Subcellular Location: Cytoplasm. Similarity: Belongs to the glutathione peroxidase family. SWISS: P07203 Gene ID: 2876 Database links: Entrez Gene: 461015 Chimpanzee Entrez Gene: 2876 Human Entrez Gene: 706732 Macaque Monkey Entrez Gene: 14775 Mouse Entrez Gene: 100438450 Orangutan Omim: 138320 Human SwissProt: Q0EFA0 Chimpanzee SwissProt: P07203 Human SwissProt: Q865R2 Macaque Monkey SwissProt: P11352 Mouse SwissProt: Q4AEI3 Orangutan Unigene: 76686 Human Unigene: 1090 Mouse Unigene: 11323 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human THP-1 cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-GPX1 (bsm-52772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Sample:
Lane 1: HepG2 cell lysate
Lane 2: THP-1 cell lysate
Primary: Anti-GPX1 (bsm-52772R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
