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Mouse Anti-CYP11A1  antibody (bsm-60352M)  
~~~促銷,代碼KT202502A~~~
~~~促銷,代碼KT202502B~~~
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產(chǎn)品編號 bsm-60352M
英文名稱 Mouse Anti-CYP11A1  antibody
中文名稱 細胞色素P450 11A1重組鼠單抗
別    名 Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase (side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HUMAN; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450 11A1 mitochondrial; Cytochrome P450 family 11 subfamily A polypeptide 1; Cytochrome P450 subfamily XIA; Cytochrome P450(scc); Cytochrome P450C11A1; mitochondrial; P450SCC; Steroid 20 22 lyase.  
研究領(lǐng)域 腫瘤  心血管  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  
抗體來源 Mouse
克隆類型 Recombinant
交叉反應(yīng) Human (predicted: Mouse,Rat)
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:20-100,IHC-F=1:400-800,IF=1:20-100,ICC/IF=1:20-100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 53/57kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CYP11A1 
亞    型 IgG1/Kappa
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

SWISS:
P05108

Gene ID:
1583

Database links:

Entrez Gene: 1583 Human

Entrez Gene: 13070 Mouse

Entrez Gene: 29680 Rat

SwissProt: P05108 Human

SwissProt: Q9QZ82 Mouse

SwissProt: P14137 Rat



產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: JAR, HepG2 Protein loading quantity: 20 μg Exposure time: 3 s Predicted MW: 52 kDa Observed MW: 52 kDa
Tissue: Human placenta Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab:SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60352M
Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60352M
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