| 產(chǎn)品編號 | bs-42464P |
| 英文名稱 | Recombinant mouse CDKN2A protein, N-GST&C-His |
| 中文名稱 | 重組小鼠抑癌基因p19蛋白 |
| 別 名 | CD2A2_HUMAN; ARF; p19ARF; Cyclin dependent kinase inhibitor 2A; INK4; MTS1; P19. |
| 理論分子量 | 47.3kDa |
| 性 狀 | Liquid |
| 濃 度 | >1mg/ml |
| 純 度 | >90% as determined by SDS-PAGE |
| 緩 沖 液 | 50mM Tris-Hcl (pH=9.0) with 10mM GSH and 0.1mM PMSF |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]. SWISS: P51480 Gene ID: 12578 p16主要功能是通過抑制CDK4而阻止細胞由G1期進入S期，使細胞增殖受到限制。用于各種惡性腫瘤如肺癌、惡黑、乳腺癌的研究。目前的研究細胞周期依賴激酶抑制p16INK4a蛋白在宮頸上皮內(nèi)病變(CIN)中作為一個新標記物. p16INK4a的過表達與HPV E7區(qū)(病毒早期蛋白即病毒致癌基因編碼區(qū))活性有密切相關(guān)性。 p16/CDKN2基因是新近發(fā)現(xiàn)的腫瘤抑制基因，已有研究表明該基因在許多腫瘤出現(xiàn)缺失、突變或重排現(xiàn)象. |
| 產(chǎn)品圖片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
